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A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. Living organisms use solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable.
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Primer required?
Hi, I have done a few tiling jobs for myself over the past few years and would rate myself as reasonably competent!:lol: I like to ask questions before going in head-first so as to limit any avoidable mistakes. So, first question for you hepful chaps is..... I am laying UFH in my flat (kitchen &...- Scholester
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- primer required usa
- Replies: 12
- Forum: UK Tiling Forum
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Applying primer - advice please :-)
Morning all! I have had a nightmare previously with tiles and thanks to your site Im making progress again lol i have a question about the Granfix acrylic primer - the instructions for plaster priming say to mix 3:1 water to primer and apply to seal. It then says apply 2 neat coats before...- Gixerguy
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- advice please primer usa
- Replies: 37
- Forum: UK Tiling Forum
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Anhydrite Primer
I'm about to lay Karndean tiles on an anhydrite screed which has hot water underfloor heating. The screed is thoroughly dry and has had the laitance removed. The tile supplier advised priming with Uzin 360 and then smoothing with Uzin NC110. My questions are:- the surface is already smooth...- floorlayer
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- adhesive anhydrite anhydrite adhesive primer screed usa
- Replies: 8
- Forum: UK Tiling Forum
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How long does primer take to dry?
Hi guys, i am starting my first floor tiling project (my own bathroom and toilet) and this site has been a great help so far. I have applied SBR bond to my plywood, but i wanted to know how long i have to wait before i can start tiling, i am using BAL rapidset flexible adhesive with 600x300...- garrybibson
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- dry how primer usa
- Replies: 22
- Forum: UK Tiling Forum
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Primer G question
It's me again. Quick question about priming. Before I started tiling a wall, I decided to have a little practice run on tiling the window sill - just to get a feel for the trowel etc. (I don't get on with a trowel incidentally - an 11 incher, and wonder if I'd be better off with a smaller...- Minnie
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- primer usa
- Replies: 14
- Forum: UK Tiling Forum
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primer
hiya peeps,just been to price a job ,old trav tiles on a concrete floor he dosent want them takin up,nor do i:thumbsup: would SBR bond be ok after cleaning,then tile,cheers steve- stevee
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- primer usa
- Replies: 7
- Forum: UK Tiling Forum
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Which Primer & Adhesive - NMP, UFH & Travertine
Hi there, I am about to undertake my first DIY tiling job on my kitchen :dizzy2: and i'm looking for some advice on which primer and tile adhesive to use. I will be laying 600x400x12mm Travertine floor tiles in a 12sqm room. I have already layed 6mm NMP over the existing wooden floor boards...- mrcandu
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- adhesive primer tile adhesive travertine ufh usa which
- Replies: 6
- Forum: UK Tiling Forum
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Addy,sealer & primer when,where & how?
For the bathroom refit i intend to take off old tiles back to plaster and tank with homelux wall & floor membrane,then if cost ok use travertine tiles 12mm thick not sure of size poss 300x450. 1.Will i need to prime the plaster before using addy to fix the wall membrane,what type of addy and...- 59peejay
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- how primer usa
- Replies: 5
- Forum: UK Tiling Forum
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Palace Tilers Primer
Hi, Just got some of Palaces Tilers Primer, from a new local tile shop. Just wondered if any of you have used it and if so is it any good? Also is it an acrylic based primer? Thanks. Vinnie.:thumbsup:- bluevin123
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- adhesives find a tiler palace palace adhesive primer tiler tilers tilers directory usa
- Replies: 5
- Forum: UK Tiling Forum
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Mapei Primer G on top of SBR (prior to Mapegum tanking)
If I have already primed all of the plastered walls with SBR (Everbuild from Tradetiler), is it advisable to apply Mapei Primer G on top of this prior to Mapegum, or do I just tank straight on top of the SBR????? Thanks - Geoff- Qwerty
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- adhesive grout mapei mapei adhesive mapei grout primer sbr tank tanking top usa waterproofing wetroom
- Replies: 9
- Forum: UK Tiling Forum
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Thermal Floor Insulating Primer v Insulation Boards
Hello good people of the board, I'm looking for some advice on my first tiling job ever. I have a kitchen (concrete floor) and an attached extension (wooden floor) to tile. I need underfloor heating at least in the kitchen to take the edge off the cold in the winter, it won't be used as the...- DannyVandal
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- boards floor floor tiling insulation insulation boards primer usa
- Replies: 4
- Forum: UK Tiling Forum
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Advice on Primer
Hi All, Can I use Febond SBR to prime walls before I tile? Thanks in Advance- rah
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- advice primer usa
- Replies: 6
- Forum: UK Tiling Forum
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Mapei Primer G
Hi, Would Mapei Primer G be a suitable primer for a concrete floor that is having porcelain tiles applied with flexible PCI adhesive. Thanks- davphi338
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- adhesive grout mapei mapei adhesive mapei grout primer usa
- Replies: 11
- Forum: UK Tiling Forum
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which adhesive with acrylic primer?
Hi guys I've heard some conflicting info can you clear it up for me please. If i have had to acrylic prime an area of a wall which adhesive should i use for ceramic tiles. Does it have to be a flexi? Thanks Richie Grout line tiling services- richie135
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- adhesive primer tile adhesive usa which
- Replies: 4
- Forum: UK Tiling Forum
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Where can I buy PCI Primer 303?
I was recommended this a while ago - I have googled for stockists but all it comes up with is people talking about it on here or the manufacturer's website... I have been fighting with the vile vinyl in my bathroom all day. The adhesive it's leaving behind is just something else. It's that...- Halo
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- adhesive can pci primer usa where
- Replies: 4
- Forum: UK Tiling Forum
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Whats the best primer to use on walls and floors???
Hi all, Just wondering what you found to be the best primer to use on floors and walls,in terms of quality and price and how far it goes. I have just run out and need to get some more. I have been using Webber PR370 its a PVA priming,sealing and bonding agent and 2.5kg watered down lasts me...- bluevin123
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- best floor floor tiling floors primer usa wall walls whats
- Replies: 12
- Forum: UK Tiling Forum
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epoxy primer
can anyone tell me the benefits of an epoxy primer - the forum recommended it when I enquired about tiling over a bitumen floor in an ensuite. also 'sbr' stands for?- mscg
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- epoxy primer usa
- Replies: 9
- Forum: UK Tiling Forum
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30L White Star and 2kg Primer G in Cheshire
I have 30 litres (3 x 10 litre pots) of Bal White Star for sale. Bought in November and stored in dry attached garage. Wasn't planning on getting porcelain tiles! I also have 2kg of Mapei Primer G spare. Again new and sealed. The stuff is in Cheshire (3 miles from Jcn 10 of M56). It is...- iaincameron
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- primer usa white
- Replies: 5
- Forum: UK Tiling Forum
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Using SBR primer on bitumen floor to stop smell
Im going to prime my solid concrete floors with SBR primer before tiling my en suite, but I also have the same crappy bitumen covering on my bedroom floor. The tiles are up and left the residue, which is really smelly (hot tarmac!). Will a coat of SBR stop this, if not then what will...- Qwerty
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- bitumen floor floor tiling primer sbr usa
- Replies: 4
- Forum: UK Tiling Forum
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