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stains

Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues) and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of disease at a microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells.
In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewing stained samples at high magnification, typically using bright-field or epi-fluorescence illumination.
Staining is not limited to biological materials, it can also be used to study the structure of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.

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  1. S

    Seals and stains on Caithness Stone / Scottish Slate

    Hey Folks, I am putting down some Caithness stone in a kitchen area. The stone is laid and now needs sealed. I have some Lithofin MN StainStop left over from a previous job and have applied this. I spoke to Lithofin themselves and they recommended I use this. I have prepared a sample...
  2. B

    Wetroom-Tiles showing stains from underneath! PLEASE HELP

    Hello We have recently installed a wetroom shower area, using a product called 'Impey' also used their recommended underfloor heating 'Devimat' We used a cream ceramic tile and the installer used Bal 2 part adhesive along with Mapei jasmine grout. Upon turning on the underfloor heating and...
  3. F

    stains and watermarks on slate tile

    hi guys and gals, Newbie to tilerforums, so Hi to everyone. Hope you can help me, I have been asked to a property where the bathroom has slate floor tiles. The bathroom had a leak and I was able to restore the grout, but the stains and watermarks on the tiles are a pain to remove. I have...
  4. S

    Cleaning stains from grey porcelain floor tiles

    My builder has just laid some black grey tiles in my bathroom, they are almost a slate effect the make is unicom starker, porcelain tiles. When the builder left I went to clean the floor, there is a large mark across the tiles near the door as if something has been spilt on them (?dirty water...
  5. F

    Damp cause of stains ?

    I've just started a job where the client has has me to replace the tiles because the tiles and grout in particular are stained. Having taken the tiles off I noticed that a small section of the plywood backing is damp :yikes: ( this is after having removed the adhesive). Firstly could this be...
  6. S

    Black Polished Porcelain stains, HELP!

    Hi all, My brother in law has just laid 18m2 400mm x 400mm black polished porcelin tiles in my kitchen, but where they have been walked on in trainers, not overly dirty just bit of dust its marked the tile and i cant get it off, its not like a stain as such its as if its imprinted on the tile...
  7. P

    polished marble flux and solder stains

    Hi there, have recently laid a polished marble floor, after laying it I gave it two coats of fila polished marble protector, it looked great, as all my work does :hurray: Unfortunatly the plumber came in after me and where he has soldered his pipes he has dropped flux and solder on the tiles it...
  8. N

    Marble with brown stains

    Hi again guys, I decided to go with the 2mm gaps in the end but Ive now laid half the bathroom floor 2 m2 with white marble 600 x 600 tiles and ia couple hours after laying light brown coffee/tar stains appeared on the edges of my tiles. I can only put it down to putting too much adhesive near...
  9. M

    Stains on reclaimed quarry tiles

    I purchased two lots of red 6" square reclaimed victorian quarry tiles a couple of years ago with the aim of laying them on my kitchen floor. One set of tiles has JCE written in the middle of the base with RUABON at the top at NWALES at the bottom. The other set has Randlay Salop written on...
  10. L

    How Do I Remove Stains?

    Hi everyone, I've just laid a light grey matt polished porcelain floor for a customer and was daft enough to use charcoal grout (never again!!!!). Some of the tiles have 'grubby' marks on them as if the muck went through the sealer (I sealed them with the right stuff the night before laying)...
  11. W

    Water stains

    Hello, I didn't pay attention when I entered but it appears that you are all in the UK, I'm in the US. I was just trying to find a way to remove water stains from the slate in my shower?:lol:
  12. T

    stains on polished porcelian tiles

    Hi, I recently layed black polished porcelian tiles. After laying them I washed the tiles with hot water to clean the dirt up etc.. As I was about to seal them before grouting. I then noticed stains on the tiles which cannot be removed as it looks like the stain is coming through the tile...
  13. C

    Stains on Porcelain tiles

    Hi Is it difficult to remove stains from polished porcelain tiles or there is no way of removing them ? Any ideas how to seal polished porcelain tiles and what to use? Any hints for installing 60x60 Porcelain tiles on concrete floors?
  14. Q

    Urine stains

    HI How do i (sorrry ) how does the wife get p*ss stains (got five year old son not me) out of bal ivory floor grout please and how soon can you tile after applying sbr. Also will i struggle to get 4sqm out of one bag of 20kg considering i'm just aspiring tiler. Cheers Steve
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